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中国科学院动物研究所周琪 男 博导
 

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研究领域

    主要从事细胞重编程机制和命运调控、干细胞多能性获得与维持等研究工作,并致力于推动再生医学和干细胞的转化应用。

招生信息

   
招生专业
071008-发育生物学
071009-细胞生物学
招生方向
干细胞与再生医学

教育背景

1994-09--1996-12   东北农业大学   博士
学历
-- 研究生 
学位
-- 博士 

工作经历

   
工作简历
2012-06~现在, 中国科学院动物研究所, 副所长
2011-12~现在, 干细胞与生殖生物学国家重点实验室(原称:计划生育生殖生物学国家重点实验室), 主任
2008-06~2012-06,中国科学院动物研究所, 所长助理
2004-05~2011-12,计划生育生殖生物学国家重点实验室, 副主任
2002-12~现在, 中国科学院动物研究所, 研究员,博士生导师
1999-03~2002-12,法国国家农业科学研究院(INRA), 博士后、项目组长
1997-03~1999-03,中国科学院发育生物学研究所, 博士后,副研究员任职资格
1994-09~1996-12,东北农业大学, 博士
社会兼职
2015-10-14-今,中华医学会医学细胞生物学分会第五届委员会, 委员
2015-08-01-今,Signal Transduction and Targeted Therapy 编委, 
2015-06-11-今,Nature partner journal Regenerative Medicine 副编辑, 
2015-05-01-今,Protein & Cell, 编委
2015-04-02-今,中国细胞生物学学会 副理事长, 
2014-11-01-今,中国动物学会第十七届理事会 常务理事, 常务理事
2014-06-25-今,中华医学会组织修复与再生分会 副主任委员, 
2014-06-19-2015-06-23,国际干细胞研究学会(ISSCR)年会组委会 委员, 
2014-01-01-今,国际干细胞组织(ISCF)主席, 
2014-01-01-今,中国细胞生物学学会干细胞研究分会 会长, 会长
2013-06-17-今,中国实验动物学会学术工作委员会 委员, 
2013-03-01-今,National Science Review, 编委
2013-02-15-今,中国实验动物学会第六届理事会 理事, 
2012-10-16-今,中国生物医学工程学会干细胞工程技术分会 副主任委员, 
2011-07-01-2016-09-30,Journal of Biological Chemistry 编委 , 
2007-01-01-今,国际干细胞组织(ISCF) 中国代表, 
2004-01-01-今,国际转基因技术学会 终身荣誉会员, 荣誉会员

教授课程

干细胞生物学
干细胞研究进展系列讲座

专利与奖励

   
奖励信息
(1) 国家自然科学奖, 部委级, 2014
(2) 中国科学院杰出科技成就奖, 院级, 2013
(3) 谈家桢生命科学创新奖, 院级, 2013
(4) 周光召基金会杰出青年基础科学奖, 院级, 2011
(5) 第十届中国科学院杰出青年, 院级, 2010
(6) 中国细胞生物学干细胞杰出贡献奖, 院级, 2010
(7) 中国侨界(创新人才)贡献奖, 院级, 2010
(8) 何梁何利基金科学与技术进步奖, 院级, 2010
(9) 第十一届“中国青年五四奖章”, 院级, 2007
(10) 第九届中国青年科技奖, 院级, 2006
(11) 第三届genOway转基因科技奖, 院级, 2004
(12) 法中科技促进会生物技术奖, 院级, 2003
(13) 法国华人青年企业家协会年度科研奖, 国家级, 2001
专利成果
( 1 ) 用于鉴定或调控细胞多能性的关键基因、microRNA、其他非编码RNA或其组合, 发明, 2015, 第 1 作者, 专利号: ZL201010142746.8
( 2 ) 一种提高体细胞重编程效率的方法, 发明, 2011, 第 1 作者, 专利号: ZL200810119651.7
( 3 ) 诱导型神经干细胞的制备方法及应用, 发明, 2014, 第 1 作者, 专利号: ZL201110164400.2
( 4 ) 无损检测细胞miRNA的表达量及确定细胞类型与状态的方法, 发明, 2013, 第 1 作者, 专利号: PCT/CN2013/075903
( 5 ) miRNA对m6A修饰水平的调控方法及其应用, 发明, 2015, 第 1 作者, 专利号: PCT/CN2015/071582
( 6 ) 无损检测细胞miRNA的表达量及确定细胞类型与状态, 发明, 2016, 第 1 作者, 专利号: ZL201210258472.8
( 7 ) 一种诱导多能干细胞的培养基及其用途, 发明, 2014, 第 1 作者, 专利号: 201410394740.8 
( 8 ) 一种用于将胚胎干细胞诱导为心肌细胞的培养基及其应用, 发明, 2015, 第 1 作者, 专利号: 201510046646.8
( 9 ) 一种诱导多能性干细胞的培养体系, 发明, 2014, 第 1 作者, 专利号: 201410109226.5

出版信息

   
发表论文
(1) Generation and application of mouse-rat allodiploid embryonic stem cells, Cell, 2016, 通讯作者
(2) Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells, Cell Research, 2016, 通讯作者
(3) Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro, Cell Stem Cell, 2016, 通讯作者
(4) Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder, Science, 2016, 通讯作者
(5) A non-invasive method to determine the pluripotent status of stem cells by culture medium microRNA expression detection, Scientific Reports, 2016, 通讯作者
(6) Co-participation of paternal and maternal genomes before the blastocyst stage is not required for full-term development of mouse embryos, J Mol Cell Biol., 2015, 通讯作者
(7) Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs, Scientific Reports, 2015, 通讯作者
(8) Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro, J Mol Cell Biol., 2015, 通讯作者
(9) Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions, Stem Cell Res Ther., 2015, 通讯作者
(10) Dynamic transcriptional symmetry-breaking in pre-implantation mammalian embryo development revealed by single-cell RNA-seq, Development, 2015, 通讯作者
(11) Derivation of a homozygous human androgenetic embryonic stem cell line, Stem Cells Dev., 2015, 通讯作者
(12) Generation of cynomolgus monkey chimeric fetuses using embryonic stem cells, Cell Stem Cell, 2015, 通讯作者
(13) Generation of fertile offspring from Kit(w)/Kit(wv) mice through differentiation of gene corrected nuclear transfer embryonic stem cells, Cell Research, 2015, 通讯作者
(14) Generation of cell-type-specific gene mutations by expressing the sgRNA of the CRISPR system from the RNA polymerase II promoters, Protein & Cell, 2015, 通讯作者
(15) Lmx1a enhances the effect of iNSCs in a PD model, Stem Cell Research, 2015, 通讯作者
(16) Maternal PCBP1 determines the normal timing of pronucleus formation in mouse eggs, Cellular and Molecular Life Science, 2015, 通讯作者
(17) Three-dimensional bio-printing, Science China Life Science, 2015, 通讯作者
(18) Generation of an LncRNA Gtl2-GFP Reporter for Rapid Assessment of Pluripotency in Mouse Induced Pluripotent Stem Cells, Journal of Genetics and Genomics, 2015, 通讯作者
(19) One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system, Cell Research, 2015, 通讯作者
(20) m6A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency, Cell Stem Cell, 2015, 通讯作者
(21) Blastomere biopsy influences epigenetic reprogramming during early embryo development, which impacts neural development and function in resulting mice, Cellular and Molecular Life Sciences, 2014, 通讯作者
(22) Generation of tetraploid complementation mice from embryonic stem cells cultured with chemical defined medium, Chinese Science Bulletin, 2014, 通讯作者
(23) Epigenetic reprogramming, gene expression and in vitro development of porcine SCNT embryos are significantly improved by a histone deacetylase inhibitor -- m- carboxycinnamic acid bishydroxamide (CBHA), Protein and Cell, 2014, 通讯作者
(24) Efficient generation of mouse ESCs-like pig induced pluripotent stem cells, Protein and Cell, 2014, 通讯作者
(25) Balancing the welfare: the use of non-human primates in research, Trends in Genetics, 2014, 通讯作者
(26) One-step generation of knockout pigs by zygote injection of CRISPR/Cas system, Cell Research, 2014, 通讯作者
(27) Genetic modification and screening in the rat using haploid embryonic stem cells, Cell Stem Cell, 2014, 通讯作者
(28) Haploid embryonic stem cells serve as a new tool for mammalian genetic study, Stem Cell Research and Therapy, 2014, 通讯作者
(29) CRISPR/Cas technology: a revolutionary approach for genome engineering, Science China Life Sciences, 2014, 通讯作者
(30) Tex101 is essential for male fertility by affecting sperm migration into the oviduct in mice., Journal of Molecular Cell Biology, 2013, 通讯作者
(31) Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems., Nature Biotechnology, 2013, 通讯作者
(32) Parthenogenetic haploid embryonic stem cells produce fertile mice, Cell Research, 2013, 通讯作者
(33) Evolutionary and Functional Analysis of the Key Pluripotency FactorOct4 and Its Family Proteins. , Journal of Genetics and Genomics , 2013, 通讯作者
(34) Derivation of androgenetic embryonic stem cells from m-carboxycinnamic acid bishydroxamide (CBHA) treated androgenetic embryos. , Chinese Science Bulletin, 2013, 通讯作者
(35) Blastomere biopsy influences epigenetic reprogramming during early embryo development, which impacts neural development and function in resulting mice. , Cellular and Molecular Life Sciences, 2013, 通讯作者
(36) Preimplantation genetic diagnosis (PGD) influences adrenal development and response to cold stress in resulting mice. , Cell Tissue Research, 2013, 通讯作者
(37) MicroRNA-323-3p Regulates the Activity of Polycomb Repressive Complex 2 (PRC2) via Targeting the mRNA of Embryonic Ectoderm Development (Eed) Gene in Mouse Embryonic Stem Cells. , Journal of Biological Chemistry, 2013, 通讯作者
(38) Generation of Transgenic Rats through Induced Pluripotent Stem Cells., Journal of Biological Chemistry, 2013, 通讯作者
(39) Direct reprogramming of setoli cells into multipotent neural stem cells by defined factors. , Cell Research, 2012, 通讯作者
(40) Rapid conversion of human ESCs into mouse ESC-like pluripotent state by optimizing culture conditions. , Protein and Cell, 2012, 通讯作者
(41) Generation of dopaminergic neurons directly from mouse fibroblasts and fibroblast-derived neural progenitors.,  Cell Research., 2012, 通讯作者
(42) PCBP1 is required for maintenance of the transcriptionally silent state in fully grown mouse oocytes., Cell Cycle, 2012, 通讯作者
(43) Androgenetic haploid embryonic stem cells produce live transgenic mice, Nature, 2012, 通讯作者
(44) Early patterning of cloned mouse embryos contributes to post-implantation development. , Developmental Biology, 2012, 通讯作者
(45) Generation of tripotent neural progenitor cells from rat embryonic stem cells. , Journal of Genetics and Genomics , 2012, 通讯作者
(46) Derivation of Germline Competent Rat Embryonic Stem Cells from DA Rats. , Journal of Genetics and Genomics, 2012, 通讯作者
(47) Induced pluripotent stem induced cells show better constitutive heterochromatin remodeling and developmental potential after nuclear transfer than their parental cells. , Stem Cell Development, 2012, 通讯作者
(48)  iPS cells generated without c-Myc have active Dlk1-Dio3 region and are capable of producing full-term mice through tetraploid complementation., Cell Research, 2011, 通讯作者
(49) Pluripotency maintenance in mouse somatic cell nuclear transfer embryos and its 3 improvement by treatment with the histone deacetylase inhibitor TSA., Cellular Reprogramming. , 2011, 通讯作者
(50) Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice. , PLoS ONE, 2011, 通讯作者
(51)  Mice generated from tetraploid complementation competent iPS cells show similar developmental features as those from ES cells but are prone to tumorigenesis. , Cell Research, 2011, 通讯作者
(52) Mouse cloning and somatic cell reprogramming using electrofused blastomeres, Cell Research , 2010, 通讯作者
(53) Viable Fertile Mice Generated from Fully Pluripotent iPS Cells Derived from Adult Somatic Cells., Stem Cell Rev., 2010, 通讯作者
(54) Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells.,  Cell Research, 2010, 通讯作者
(55) Somatic nucleus reprogramming is significantly improved by m- carboxycinnamic acid bishydroxamide (CBHA), a histone deacetylase inhibitor.,  J Biol Chem. , 2010, 通讯作者
(56) Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. , J Biol Chem., 2010, 通讯作者
(57) Nuclear transfer-derived epiblast stem cells are transcriptionally and epigenetically distinguishable from their fertilized-derived counterparts. , Stem Cells., 2010, 通讯作者
(58) Efficient and rapid generation of induced pluripotent stem cells using alternative culture medium. , Cell Research, 2010, 通讯作者
(59) HSPC117 deficiency in cloned embryos causes placental abnormality and fetal death. , Biochemical and Biophysical Research Communications, 2010, 通讯作者
(60) Transgenic rhesus monkeys produced by gene transfer into early-cleavage–stage embryos using a simian immunodeficiency virus-based vector. , ProcNatlAcadSci USA, 2010, 通讯作者
(61) Production of mice using iPS cells and tetraploid complementation. , Nat Protoc., 2010, 通讯作者
(62) Derivation of embryonic stem cells from Brown Norway rats blastocysts. , Journal of Genetics and Genomics, 2010, 通讯作者
(63)  , Derivation and long-term culture of human parthenogenetic embryonic stem cells using human foreskin feeders., J Assist Reprod Genet., 2010, 通讯作者
(64) Decreased cofilin1 expression is important for compaction during early mouse embryo development. , BiochimBiophysActa., 2010, 通讯作者
(65) iPS cells produce viable mice through tetraploid complementation. , Nature , 2009, 通讯作者
(66) Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system. , Reprod Biomed Online, 2009, 通讯作者
(67) )Human parthenogenetic embryonic stem cells: one potential resource for cell therapy. , Sci China C Life Sci., 2009, 通讯作者
(68)  A modified culture method significantly improves the development of mouse somatic cell nuclear transfer embryos.,  Reproduction , 2009, 通讯作者
(69) Evaluation of blastomere biopsy using mouse model indicates the potential high-risk of neurodegenerative disorders in the offspring. , Mol Cell Proteomics, 2009, 通讯作者
(70) Isolation and characterization of liver epithelial progenitor cells from normal adult rhesus monkeys (Macacamulatta). , Cell Research, 2009, 通讯作者
(71) Assessment of the developmental competence of human somatic cell nuclear transfer embryos by oocyte morphology classification., Hum Reprod., 2009, 通讯作者
(72) Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos. , Cloning Stem Cells, 2008, 通讯作者
(73) Protein expression profile of the mouse metaphase-II oocyte., J Proteome Res. , 2008, 通讯作者
(74) Dissecting Signaling Pathways That Govern Self-renewal of Rabbit Embryonic Stem Cells.,  J Biol Chem., 2008, 通讯作者
(75) Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts. , Chinese Science Bulletin , 2008, 通讯作者
(76) Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season. , Theriogenology, 2008, 通讯作者
(77) Derivation of human embryonic stem cell lines from parthenogenetic blastocysts. , Cell Research , 2007, 通讯作者
(78)  Superovulatory response to a low dose single-daily treatment of rhFSH dissolved in polyvinylpyrrolidone in rhesus monkeys. , Am J Primatol., 2007, 通讯作者
(79) Effects of rhFSH dose on ovarian follicular response, oocyte recovery and embryo development in rhesus monkeys. , Theriogenology , 2007, 通讯作者
(80) Epigenetic marks in cloned rhesus monkey embryos: comparison with counterparts produced in vitro., Biol Reprod. , 2007, 通讯作者
(81) A comparative approach to somatic cell nuclear transfer in the rhesus monkey.,  Hum Reprod. , 2006, 第 1 作者
(82) Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells. , Stem Cells , 2005, 通讯作者
(83) Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells. , Stem Cells, 2005, 通讯作者
(84) Generation of fertile cloned rats by regulating oocyte activation. , Science, 2003, 第 1 作者
(85)  Nuclear Transfer: New Advances and Quandaries. , Reproductive Biology and Endocrinology, 2003, 通讯作者
(86) Developmental potential of mouse embryos reconstructed from metaphase embryonic stem cell nuclei. , Biol Reprod., 2001, 第 1 作者
(87) A simplified method for the reconstruction of fully competent mouse zygotes from adult somatic donor nuclei. , Cloning, 2000, 第 1 作者
发表著作
( 1 ) 神秘的克隆, Clone, 中国教育科学出版社, 1997-08, 第 1 作者
( 2 ) 干细胞实验指南, Handbook: Stem Cell Biology, 中央广播电视大学出版社, 2015-07, 第 1 作者

科研活动

   
科研项目
( 1 ) 中国科学院战略性先导专项A类——干细胞与再生医学研究, 主持, 部委级, 2011-01--2015-12
( 2 ) 灵长类细胞多能性的研究, 主持, 国家级, 2012-01--2016-08
( 3 ) DNA与RNA甲基化表观修饰对单倍体胚胎干细胞多能性与所产生个体发育能力的影响和作用机制研究, 主持, 国家级, 2014-01--2016-12
( 4 ) RNA甲基化修饰对体细胞重编程的调控作用及机制研究, 主持, 国家级, 2015-01--2018-12
参与会议
(1)Haploid embryonic stem cells: a new tool for genetic modification and screening   周琪   2015-12-18
(2)Progress in Generating Primate-Pig Chimera in China   周琪   2015-11-06
(3)Clinical grade stem cell lines and banking   第十六届北京分析测试学术报告会暨展览会(BCEIA 2015)   周琪   2015-10-28
(4)Generation and application of pluripotent stem cells - from bench to bedside   周琪   2015-10-21
(5)The progresses and perspectives of stem cell research and regenerative medicine   周琪   2015-10-19
(6)Haploid stem cells as a new tool for genetic and developmental studies   2015亚太发育生物学国际研讨会   周琪   2015-09-12
(7)中国干细胞研究与转化的机遇与挑战   第八届北京干细胞论坛   周琪   2015-07-11
(8)Haploid embryonic stem cells as a new tool for genetic modification and screening   周琪   2015-05-19
(9)动物疾病模型在生殖医学领域的应用   PGD技术交流会   周琪   2014-12-19
(10)Translation of Stem Cell Research: from Discoveries to Application   第七届广州国际干细胞与再生医学论坛   周琪   2014-12-17
(11)干细胞研究:从基础走向临床   东北农业大学第六届“生命科学论坛”暨第四届“秦鹏春教育基金”系列学术讲座   周琪   2014-12-13
(12)细胞命运调控及转化应用   第二届海峡两岸生命科学论坛   周琪   2014-12-02
(13)对生殖生物学与生殖健康领域未来发展的一些思考   第128期双清论坛“生殖生物学研究的挑战和对策”   周琪   2014-11-25
(14)Basic Research and Potential Application of Stem Cell   周琪   2014-11-02
(15)干细胞在遗传筛选和疾病研究中的作用   全球华人遗传学大会暨全国第十三次医学遗传学学术会议   周琪   2014-10-30
(16)Animal Models for Stem Cell Research and Application: from Rodents to Non-human Primates   周琪   2014-10-13
(17)Pre-clinical Research of Stem Cell Therapy   周琪   2014-08-17
(18)利用基因修饰技术制备人类疾病大动物模型   第三届“模式生物与人类健康”发育遗传学全国学术研讨会   周琪   2014-07-18
(19)The Pluripotency of Haploid Embryonic Stem Cells and Its Application   周琪   2014-05-29
(20)基因编辑:细胞和动物模型的新视野   香山会议——基因组编辑前沿技术:应用、生物安全与伦理   周琪   2014-05-10
(21)利用基因编辑技术制备细胞与动物模型   遗传学与表观遗传学前沿暨第三届中国青年遗传学家论坛   周琪   2014-04-12
(22)Studies of Pluripotent Stem Cells and Transgenic Animal Models   周琪   2014-04-11
(23)Genome editing and animal models   周琪   2014-02-12
(24)Research and application of haploid embryonic stem cells   第六届广州国际干细胞与再生医学论坛   周琪   2013-12-18
(25)利用单倍体干细胞进行基因筛选与修饰   第五届东北农业大学“生命科学论坛”暨第三届“秦鹏春教育基金”系列学术讲座   周琪   2013-12-07
(26)Stem Cell Research and Clinical Application   周琪   2013-11-10
(27)Stem cell research and application in China   第二十届东亚医学和生物科学联合研讨会   周琪   2013-11-06
(28)The reconstructed tissue and life from stem cells   干细胞医学仿生学研讨会   周琪   2013-08-13
(29)孤雄单倍体干细胞——获得基因修饰动物模型的新工具   全国动物生物技术学术研讨会   周琪   2013-07-24
(30)Cell Fate Determination and Regulation – the Hope of Medical Revolution   全球华人生物科学家大会   周琪   2013-07-19
(31)器官与生命的再造   香山科学会议20周年学术报告会   周琪   2013-07-16
(32)Stem cells, the seeds for tissue regeneration   周琪   2013-07-02
(33)Cell programming, reprogramming, and pluripotency   第一届细胞死亡与疾病研讨会暨第四届中英“细胞死亡、干细胞与癌症”国际研讨会   周琪   2013-05-08
(34)干细胞——新医学革命的核心   第六届全国组织工程与再生医学大会   周琪   2013-04-19
(35)干细胞、iPS和动物克隆   香山科学会议第456次学术讨论会   周琪   2013-04-03
(36)配子来源的干细胞和干细胞来源的配子   生殖医学年会   周琪   2013-03-09
(37)From Haploid Stem Cells to Transgenic Mice   第十一届国际转基因技术大会   周琪   2013-02-26
(38)生殖细胞、干细胞与生殖生物技术的未来   北京生殖年会   周琪   2012-02-24
(39)Progress of Stem Cell Research and Potential Application in Regenerative Medicine   第十四届中美前沿科学研讨会   周琪   2011-11-05
(40)中国干细胞研究发展报告   第9届国际干细胞组织年会   周琪   2011-10-04
(41)干细胞及再生医学研究进展及潜在应用   2011诺贝尔奖获得者北京论坛   周琪   2011-09-28
(42)Production of viable fertile offspring from induced pluripotent stem cells   国际第七届台湾干细胞学会年会   周琪   2011-09-28
(43)Pluripotent stem cells and the potential application in regenerative medicine   美洲华人生物科学学会第十三届国际学术讨论会   周琪   2011-07-25
(44)诱导多能性细胞的重编程及分子识别标识   第六届亚太地区细胞生物学(APOCB)学术大会   周琪   2011-02-24
(45)Reprogramming and biomarkers of pluripotent stem cells   第三届广州国际干细胞与再生医学论坛   Qi Zhou   2010-12-19
(46)重编程:从体细胞到多能性细胞   新加坡干细胞学会2010研讨会   周琪   2010-11-01
(47)多能性细胞研究进展与再生医学发展   2010发育过程中健康与疾病的研究学术研讨会   周琪   2010-10-20
(48)由iPS细胞到四倍体补偿动物的出生   第四届苏兰拉里理工大学干细胞研讨会   周琪   2010-07-26
(49)人类体细胞核移植的研究现状   2. 加利福尼亚再生医学研究所/英国医学研究理事会人类体细胞核移植研讨会   周琪   2010-06-14
(50)From iPS to live birth: update in iPS research   第十届辅助生殖技术临床与实验协会国际研讨会   周琪   2010-05-05

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